Optimizing the efficacy and safety of engineered lymphocytes requires an in-depth understanding of cellular states and state changes: These phenomena have been investigated in other cellular systems based on a sophisticated zoo of bioinformatical tools, that for example is employed in Human Cell Atlas porjects. We propose to bring together state-of-the-art bioinformatics analysis and mathematical modeling to monitor and improve the in vivo functionality of the engineered lymphocytes.
CAR-T perturbations, (v) quantify differentiation, migratory and proliferative behavior, and (vi) compare the developmental trajectories of differently engineered lymphocytes.
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